Angiogenesis 2D Assay
The standard 2D assay commonly used for angiogenesis studies involves an 11 day incubation. Experiments are initiated and the microplate is placed inside the incubator for the duration of the experiment, removed at the end of the 11 days when the cells are fixed and tubules present stained. Generally the tubules formed are measured using a manaul or semi-automated process.
Cell-IQ® has been used successfully to image the formation of tubules during the 11 day incubation period and quantify the stained tubules formed at the end of the experiment providing a rapid automated procedure for the researcher. Below is an example of a phase contrast image with stained tubules and the corresponding analysed image using the Cell-IQ Analyser™ Software, where the tubule length has been measured.
Phase contrast image with stained tubules Analysed image where tubules are quantified